ELISA kits can be used to quickly and accurately identify and quantify targets in samples and cultures. There are many options available, including competitive ELISA and sandwich ELISA, as well cell-based ELISA Kits that can be used to identify many targets. These ELISA kits come with pre-coated plates that are coated with standards, buffers, capture/detection antibodies and other reagents.
To ensure precision, accuracy and reproducibility, each kit is subject to fit-for-purpose validation. ELISA (enzyme linked immunosorbent assay), is a plate-based technique that can detect and quantify soluble substances, such as peptides and proteins. You can buy high-quality ELISA kits via Boster Bio.
The same technology can also be called enzyme immunoassay (EIA) or other names such as enzyme-linked immunosorbent assay (ELISA). An ELISA uses the target macromolecule (antigen) to immobilize on a microplate and then complex it with an antibody that is linked with a reporter enzyme. The reporter enzyme's activity is measured by incubating with the substrate.
This produces a measureable product. An ELISA requires a specific antibody-antigen interaction to be effective. The enzyme-linked immunosorbent assay is a powerful tool for detecting and quantifying a particular protein in a complex mixture. It allows for analysis of proteins immobilized in microplates with specific antibodies.
ELISAs are usually performed on 96-well and 384-well polystyrene plate, which passively bind proteins and antibodies. This binding and immobilization makes ELISAs simple to create and use. The ELISA reactants are immobilized on the microplate surface, making it easier to distinguish bound material from non-bound materials during the assay.
ELISA is a powerful tool to measure specific analytes in a crude preparation because it can be used with high-affinity antibodies as well as washed away any non-specific bound material. There are many substrates that can be used in ELISA using an AP or HRP conjugate.